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KMID : 0614620130610010009
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Korean Journal of Gastroenterology
2013 Volume.61 No. 1 p.9 ~ p.16
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Metabolomic Study of a Diagnostic Model for the Metabolites of Stool Fat
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Lee Choong-Hwan
Kim Ji-Young
Ahn Soo-Young
Lee Sun-Young
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Abstract
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Background/Aims: Metabolomics is a powerful tool for measuring low-molecular-weight metabolites in an organism at a specified
time under specific environmental conditions. The aim of this study was to determine the usefulness of metabolomics in
identifying the metabolites in stool-fat-positive specimens, and to establish whether the results could be used to predict the
long-term prognosis.
Methods:Fecal specimens were collected from 52 subjects with bowel habit change. The subjects were accessed using Rome
III questionnaires and Bristol stool scale form, and followed after three years. The feces samples were centrifuged and the
resulting extracts reconstituted for liquid chromatography/mass spectrometry analysis. The datasets were autoscaled, log-transformed,
and mean-centered in a column-wise fashion prior to principal-components analysis and partial least-squares-discrimination
analysis modeling.
Results:Fecal samples from 10 of the 52 patients gave a positive stool-fat result of 30-100 μm; those of the remaining
42 contained neither fatty acids nor neutral fats. The peak intensities of lithocholic acid (p=0.001), lysophosphatidyl ethanolamine
(lysoPE) 16:0 (p=0.015), and lysoPE 18:1/0:0 (p=0.014) were correlated with the size of the fatty acid. Subjects with positive
stool-fat result showed higher score in Bristol stool scale form than those with negative stool-fat result at initial (p=0.040)
and after three years (p=0.012).
Conclusions:The metabolomic assay of stool fatty acid revealed mainly lysoPEs and lithocholic acid. The size of the fatty
acid was correlated with higher concentrations of lysoPEs and lithocholic acid in stool-fat-test-positive specimens and related
to loose stool even after three years of follow-up period.
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KEYWORD
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Bristol stool scale form, Fatty acid, Metabolomics, Stool fat
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